rabbit polyclonal anti pcna primary antibody  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

doi: 10.64898/2026.01.27.701991

Figure Lengend Snippet: (A) Schematic of a sagittal view of the zebrafish brain highlighting the proximate location of the diencephalic posterior tubercular nucleus (PTN) and innervation of the Mauthner cells (M-cell). (B) Confocal projections of PCNA expression in a representative communal, dominant, and subordinate Tg( dat :egfp) zebrafish. Merged channel also shows DAPI nuclear staining in blue. Arrowheads point to PCNA expressing PTN somata. Scale bar = 20 μm. (C) Summary graph of the fraction of PTN neurons that express PCNA. Symbols represent individual samples; bars represent averages, error bars represent SEM. Dashed lines show pairwise comparison connecting each dominant to its subordinate counterpart. (Com n=6, Dom n= 8, Sub n= 8; One-way ANOVA, P=0.0029 , One-way ANOVA, Tukey’s Multiple Comparison post-test).

Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

Techniques: Expressing, Staining, Comparison

Journal: bioRxiv

Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

doi: 10.64898/2026.01.27.701991

Figure Lengend Snippet: (A) PC1 and PC2 are the first and the second principal components excluding social isolates. (B) Eigenvalues of the principal components excluding social isolates showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. (C) PC1 and PC2 are the first and the second Principal components excluding PCNA as a component. (D) Eigenvalues of the principal components excluding PNCA as a component showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. In A and C, points represent individual samples (n= 6 per social condition), different colors and symbols represent different groups. Ellipses represent 68% confidence intervals of core regions. Loading vectors represent original variables, the directions of arrows represent correlation between original variable and principal components, lengths represent association of original data to principal components. In B and D components are listed in descending order (highest to the lowest).

Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

Techniques:

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Poldip2 Knockdown Inhibits Vascular Smooth Muscle Proliferation and Neointima Formation by Regulating the Expression of PCNA and p21

doi: 10.1038/s41374-018-0103-y

Figure Lengend Snippet: Poldip2 and PCNA are co-expressed in early stages of human atherosclerosis. Sections of human coronary arteries were classified into 3 atherosclerotic stages using H&E staining, as described in Methods: stage II (a-d), stage IV (e-h) and stage VI (i-l). Immunofluorescence was used to detect Poldip2 (green) and PCNA (red). Poldip2 is more highly expressed in the endothelial layer of stage II arteries (indicated by arrows) than in neointima and media, but is apparent throughout the vessel wall in stage IV and VI arteries. PCNA is highly expressed in the medial and intimal layers of stage II and IV arteries, but is limited in stage VI arteries. Pearson coefficients, indicating colocalization of Poldip2 and PCNA were high in all analyzed images: stage II: 0.66±0.02 (n=28); stage IV: 0.68±0.02 (n=22); stage VI: 0.62±0.04 (n=22), suggesting that Poldip2 is upregulated in proliferating cells. Arterial layers are indicated by letters: I (intima), M (media). Nuclei were counterstained in blue with DAPI. Images are representative of sections from at least 4 patients for each stage of atherosclerosis. Scale bars: 50μm.

Article Snippet: Following blocking, sections were incubated with a rabbit polyclonal anti-PCNA primary antibody (1:100 in 3% BSA, Abcam) at 4°C overnight.

Techniques: Staining, Immunofluorescence

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Poldip2 Knockdown Inhibits Vascular Smooth Muscle Proliferation and Neointima Formation by Regulating the Expression of PCNA and p21

doi: 10.1038/s41374-018-0103-y

Figure Lengend Snippet: Expression of PCNA and p21 after intraluminal injury of the femoral artery. Right femoral arteries were harvested from mice two weeks after induction of intraluminal injury by insertion of a guide wire. PCNA (A) and p21 (B) were detected by immunofluorescence (red) as described in the methods section and nuclei were counter stained with DAPI (blue). Autofluorescence is shown in green. While the numbers of cells positive for PCNA were decreased, those expressing p21 were increased in arteries from Poldip2 +/− vs. wild type mice. Images are representative of sections from at least 3 animals of each genotype. Scale bars are 50 μm.

Article Snippet: Following blocking, sections were incubated with a rabbit polyclonal anti-PCNA primary antibody (1:100 in 3% BSA, Abcam) at 4°C overnight.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Poldip2 Knockdown Inhibits Vascular Smooth Muscle Proliferation and Neointima Formation by Regulating the Expression of PCNA and p21

doi: 10.1038/s41374-018-0103-y

Figure Lengend Snippet: Poldip2 knockdown normalizes cell cycle markers following vascular injury. Intraluminal injury was induced by insertion of a guide wire into the right femoral artery in mice. Arteries were harvested 2 weeks after surgery and pooled samples from 2–4 animals were processed for protein analysis using Western blotting. In injured vessels, PCNA was reduced (by 29±16%, n=5, P<0.05) and p21 was increased (by 74±27%, n=3, P<0.05) in arteries from Poldip2 +/− vs. wild type mice. Thus, Poldip2 knockdown appears to normalize cell cycle markers affected by neointimal proliferation. Equal loading between lanes was verified using β-actin. Data from injured arteries are representative from 3–5 independent experiments. Two lanes of uninjured arteries from a single experiment are also included for comparison.

Article Snippet: Following blocking, sections were incubated with a rabbit polyclonal anti-PCNA primary antibody (1:100 in 3% BSA, Abcam) at 4°C overnight.

Techniques: Knockdown, Western Blot, Comparison

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Poldip2 Knockdown Inhibits Vascular Smooth Muscle Proliferation and Neointima Formation by Regulating the Expression of PCNA and p21

doi: 10.1038/s41374-018-0103-y

Figure Lengend Snippet: Poldip2 knockdown affects cell cycle markers in cultured VSMC. VSMC were transfected with control (siControl) or Poldip2 (siPoldip2) siRNA and processed for Western blotting after the indicated number of days in culture in the presence of 10% calf serum. Membranes were probed for Poldip2 (A), p21 or PCNA (B) using corresponding primary antibodies. Equal loading was verified using β-actin (A) or β-tubulin (B) antibodies. (C and D) Quantification of results from panel B. At days 2 and 4, p21 and PCNA were significantly upregulated and downregulated, respectively, by siPoldip2 (*P<0.05 vs. siControl). Bars represent the average ± SEM of data from 5 (C) and 7 (D) independent experiments. The effectiveness of siPoldip2 treatment from one experiment is shown (A).

Article Snippet: Following blocking, sections were incubated with a rabbit polyclonal anti-PCNA primary antibody (1:100 in 3% BSA, Abcam) at 4°C overnight.

Techniques: Knockdown, Cell Culture, Transfection, Control, Western Blot

Journal: Molecular medicine reports

Article Title: Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma.

doi: 10.3892/mmr.2016.5050

Figure Lengend Snippet: Figure 1. Effect of Z. seed oil on the lung histopathology of OVA‑induced asthma and inflammation. Representative (A‑C) hematoxylin and eosin (H&E) and (D‑F) proliferating cell nuclear antigen (DAB and H&E) staining of lung sections following 24, 48 and 72 h, and 7 and 14 d treatment (x200 magnification). (A and D) Vehicle control, (B and E) OVA‑treated and (C and F) OVA + Z. seed oil‑treated group. (G) Inflammation scores in the lung tissue of vehicle‑ , OVA‑ , and OVA + Z. seed oil‑treated mice. Data are presented as the mean ± standard deviation (n=6). *P<0.05 vs. the OVA‑treated group. d, days; OVA, ovalbumin; Z. seed oil, Zanthoxylum bungeanum seed oil.

Article Snippet: Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in methanol (Li’an-Long Bohua Medical Chemical Co., Ltd.) for 5 min, and non‐specific binding was blocked with 1% bovine serum albumin (Chembase Bio; Beijing, China) in phosphate-buffered saline (PBS; EUROIMMUN AG, Luebeck, Germany) for 1 h. Sections were incubated with rabbit polyclonal proliferating cell nuclear antigen (PCNA) primary antibody (1:600; BIOSS, Beijing, China; cat. no. bS-0941R) overnight at 4 ̊C.

Techniques: Histopathology, Staining, Control, Standard Deviation